The C57BL/6J mice were assigned at random to two groups; the experimental group was fed cholesterol-rich diets; the control group received normal mouse chow. Mice receiving normal diets were completely free of atherosclerotic lesions. In mice fed cholesterol-rich diets for 16 weeks, the lesions of the aorta and coronary arteries were observed by laser scanning confocal microscopy (LSCM). The typical atherosclerotic plaques, which consisted of amounts of foam cells. were formed near the aortic valves.Roberts & Theompson remarks were 8. 0 ±1. 2. The atherosclerotic lesions were verey similar pathologically to those in the human. The plasma total and free cholesterol levels increased progressively. The obtained results suggested that an atherosclerotic murine model be successfully established. It may provide an avallable experimental model for further investigation into the molecular mechanism of atherosclerosis and screening of antiatherosclerotic drugs.