通心络对实验性家兔主动脉粥样斑块内血管内皮生长因子表达的影响
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Effects of Tongxinluo on the Expression of Vascular Endothelial Growth Factor in Rabbit Aortic Atherosclerotic Plaques
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    摘要:

    通过构建实验性家兔主动脉粥样硬化模型,观察通心络及辛伐他汀对血清氧化型低密度脂蛋白、一氧化氮、一氧化氮合酶和乳酸脱氢酶及血管形态学的影响,并采用免疫组织化学、逆转录—聚合酶链反应及WesternBlot蛋白印迹等方法,从不同水平观察通心络及辛伐他汀对动脉粥样斑块血管内皮生长因子表达的影响。结果发现,通心络减少动脉粥样斑块血管内皮生长因子表达,降低血清氧化型低密度脂蛋白及乳酸脱氢酶浓度,提示通心络可增加粥样斑块稳定性,延缓动脉粥样硬化的进程。

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    Aim To investigate the effects of Tongxinluo and simvastatin on the expression of vascular endothelial growth factor (VEGF) in rabbit aortic atherosclerotic plaques. Methods The rabbit aortic atherosclerotic model was developed by high cholesterol feeding. The concentrations of oxidized low density lipoprotein (ox-LDL), nitric oxide (NO), nitric oxide synthase (NOS) and lactate dehydrogenase (LDH) were determined. The levels of VEGF protein in atherosclerotic plaques were studied by means of immunohistochemistry and Western Blot. The expressions of VEGF mRNA were studied by means of reverse transcription polymerase chain reaction. Results Tongxinluo and simvastatin decreased the levels of VEGF protein in atherosclerotic plaques and the concentrations of ox-LDL and LDH. Conclusion Tongxinluo and simvastatin can decelerate the progression of atherosclerosis and protect the function of endothelial cells.

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张路,吴宗贵,廖德宁,潘晓明.通心络对实验性家兔主动脉粥样斑块内血管内皮生长因子表达的影响[J].中国动脉硬化杂志,2004,12(2):177~181.

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  • 收稿日期:2003-08-30
  • 最后修改日期:2003-12-15
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