Aim To investigate the single nucleotide polymorphism (SNP) SNP4 and S19W of the apolipoproteiin AⅤ (ApoAⅤ) gene possible association with serum lipids in Hunan Miao population. Methods 241 normal subjects were collected to detemine the genotypes by using polymerase reaction restriction fragments length polymorphisms. Results Only one genotype, 56C/C of S19W was found in 241 subjects. Three genotypes, -12238T/C, T/T and C/C of SNP4 were found in 241 subjects. Allelic frequencies of T and C were 0.45 and 0.55. The level of serum triglyceride (TG) in subjects with T/C genotype were significantly higher than those in subjects with other genotypes (T/C vs T/T vs C/C: 2.15±1.52 mmol/L vs 1.63±0.89 mmol/L vs 1.41±0.84 mmol/L, p<0.05). The level of serum low density lipoprotein (LDL) in subjects with T/C genotype was significantly higher than those in subjects with other genotypes too (T/C vs T/T vs C/C: 2.93±0.84 mmol/L vs 2.44±0.70 mmol/L vs 2.37±0.85 mmol/L, p<0.05). Conclusions S19W is not a polymorphism place in ApoAⅤ gene of Hunan Miao population. The polymorphism of SNP4 in ApoAⅤ gene is associated with level of serum lipids in Hunan Miao population. T/C type might be associated with higer level of serum TG and LDL (p<0.05).