Aim To approach Zhixinkang possible regulation mechanism for atherosclerosis (As) foam cell, and to investigate the effect of Zhixinkang and Lovastatin on CD36 mRNA in foam cell originated from vascular smooth muscle cell (VSMC). Methods The cultured VSMC were loaded with oxidize low density lipoprotein (ox-LDL) and interfered in Zhixinkang and Lovastatin. The expression of CD36 mRNA were detected with flow cytometer and In Situ hybridization and analyzed with statistics. Results Zhixinkang and Lovastatin could decrease CD36 mRNA expression than that in the isotonic Na chloride group and the blank group. Moreover, CD36 mRNA expression in Zhixinkang group was different from that in Lovastatin group. Conclusion Zhixinkang could decrease CD36 mRNA expression, which was superior to Lovastatin. Zhixinkang had the effect of tonifying qi and promoting blood, neutralizing poison and resolving turbid in VSMC, which was one of mechanism to inhibit the multiplication of VSMC and restrain the formation of foam cell.