Aim To establish the method of detecting functional lipoprotein(a),and evaluate it,then compare its effect with creatine kinase isozyme at the diagnosis and detection of the acute myocardial infarction.Methods The level of lipoprotein(a) and lipoprotein(a) combining to polyphosphate lysine at preoperative and postoperative 4 h,8 h,12 h,24 h,48 h and 72 h,4～9 days and 10～12 days points were detected respectively in acute myocardial infarction and unstable angina patients by enzyme-linked immunosorbent assay and immune turbidimetric assay.Results The change of lipoprotein(a) level was lower(P>0.05);However,the change of lipoprotein(a) combining to polyphosphate lysine was larger in acute myocardial infarction(P<0.05);The precision and accuracy of the method is better(>90%);The diagnostic value was more superior than creatine kinase isozyme.Conclusion The level of lipoprotein(a) detected by traditional methods fail to associate with the cardiovascular disease;The method that detecting the level of lipoprotein(a) combining to polyphosphate lysine can diagnose and monitor cardiovascular disease,and the method can be used to research the pathological mechanism of lipoprotein(a).