AimTo introduce a rapid and efficient model of myocardial infarction (MI) without the requirement of ventilation in the mouse and comprehensively describe its details.Methods40 Male BABL/C6 mice were divided into 2 groups: new method MI (15) and new method Sham (25).In the new method, hearts of total 40 mice were manually exposed through a small incision of the left fourth intercostal space without intubation. In group of MI， the left anterior descending coronary artery was ligated.Hearts from group of Sham were without ligation.This novel MI procedure is rapid, with an average procedure time of 60±15 seconds.We compared the difference between MI and Sham post 28days with analysis of echocardiography, morphology and pathology.ResultsSurvival rate of group MI was 80% and group Sham was 100% 28 days after surgery with our method.The echocardiography showed that the heart function of the mice with 28-day post-MI was markedly impaired.The LVIDd and LVIDs were significantly increased from 3.52±0.10 mm and 2.60±0.19 mm to 4.65±0.08 mm and 4.36±0.13 mm (p< 0. 0001)，while LVEF and LVFS were decreased significantly 66.70%±1.41% and 35.90%±1.01% vs 29.70%±1.64% and 14.20%±0.80%，p<0.0001，respectively.Anatomical analysis of hearts with 28-day post-MI showed enlarged left ventricles and thin ventricular walls.