丹红、C反应蛋白与基质金属蛋白酶1表达的关系
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Relationship Among Dan Hong, C-reactive Protein and Expression of Matrix Metalloproteinase-1
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    摘要:

    目的探讨中药丹红、C反应蛋白与THP-1源性巨噬细胞基质金属蛋白酶1表达的关系。方法采用佛波醇将THP-1细胞诱导分化成巨噬细胞后,用酶联免疫吸附法测定细胞基质金属蛋白酶1蛋白表达峰值时间。再将其分为对照组、C反应蛋白干预组、丹红注射液干预组。酶联免疫吸附法测定不同组别细胞培养上清液中基质金属蛋白酶1蛋白浓度,再用逆转录聚合酶链反应测定细胞不同时间和C反应蛋白干预组基质金属蛋白酶1的mRNA表达。并将基质金属蛋白酶1蛋白浓度和mRNA表达进行比较分析。结果显微镜下观察到细胞株THP-1在佛波醇诱导下转变为巨噬细胞,在24 h细胞的上清液基质金属蛋白酶1蛋白和mRNA表达到峰值。与对照组相比,C反应蛋白干预组中基质金属蛋白酶1蛋白和mRNA表达升高,5、10、25、50 mg/L C反应蛋白干预时基质金属蛋白酶1表达逐渐增高(p<0.05),呈剂量依赖性。与对照组相比,丹红注射液干预组中基质金属蛋白酶1的表达降低,0、200、400、800、1 600、3 200 mL/L丹红干预时基质金属蛋白酶1的表达减低(p<0.05),呈节段性依赖性递减,且0 mg/L和50 mg/L C反应蛋白干预下两组干预基质金属蛋白酶1表达无差异(p>0.05)。结论THP-1细胞分化成巨噬细胞后,在24 h基质金属蛋白酶1蛋白和mRNA表达最高,C反应蛋白能促进THP-1源性巨噬细胞表达基质金属蛋白酶1,丹红能抑制基质金属蛋白酶1分泌,在有C反应蛋白干预基质金属蛋白酶1表达无显著差别。

    Abstract:

    AimThe aim of this study was to investigate the relation of traditional Chinese drug Dan Hong, C-reactive protein (CRP) and matrix metalloproteinase-1 (MMP-1).MethodsHuman THP-1 cells were induced to differentiated into macrophages with phorbol-12 myristate 13-acetate (PMA).Enzyme-linked immunosorbent assay (ELISA) was used to determine the macrophages secretes MMP-1 in peak time.Thereafter, cells were divided into three groups: control group, CRP group, Dan Hong injection group, ELISA was applied to test the MMP-1 concentration in the cell supernatant of three groups, and then MMP-1 mRNA expression at different time was tested by reverse transcription polymerase chain reaction (RT-PCR) in CRP group.At last, the protein concentration and mRNA expression of MMP-1 were analyzed.ResultsTHP-1 cell induction into macrophages was observed under the microscope, the peak of MMP-1 secretion was in 24-hour.Compared with the control group, protein and mRNA expression of MMP-1 in CRP group was up-regulated gradually with 5 mg/L, 10 mg/L, 25 mg/L, 50 mg/L CRP.The result exposed that MMP-1 level was markedly elevated in a concentration dependent fashion (p<0.05).Compared with the control group, MMP-1 ex-pression in Dan Hong injection group was lower than the control group.MMP-1 expression in Dan Hong injection group was down-regulated segmentally and dependently with 200 mL/L, 400 mL/L, 800 mL/L, 1 600 mL/L, 3 200 mL/L Dan Hong injection, but MMP-1 expression by Dan Hong injection intervention has no significance whether with CRP (50 mg/L) or not (p>0.05).ConclusionsCRP promotes the expression of MMP-1 in THP-1 derived macrophages.Dan Hong can attenuate the MMP-1 secretion in interval, and there was no difference with or without CRP.

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陈东,严激,潘文博,钟万生.丹红、C反应蛋白与基质金属蛋白酶1表达的关系[J].中国动脉硬化杂志,2012,20(8):714~718.

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  • 收稿日期:2011-08-22
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