Aim To observe the effect and mechanism of soluble epoxide hydrolase inhibitor t-AUCB regulating murine endothelial progenitor cells’ function. Methods Separated by density gradient centrifugation to obtain mouse bone marrow progenitor cells, different concentrations of t-AUCB and peroxisome proliferator activated receptorγ （PPARγ） blocker GW9662 pre-intervented endothelial progenitor cells, after that endothelial progenitor cell proliferation, adhesion, migration, angiogenesis ability were detected in vitro. Results From 0～100 μmol/L, as the concentration increases, t-AUCB can increase endothelial progenitor cell proliferation, adhesion, migration, angiogenesis ability in vitro. Conclusion Soluble epoxide hydrolase inhibitor t-AUCB involved in forward regulating endothelial progenitor cell’ function, and its function is associated with activation of PPARγ on endothelial progenitor cell.