Aim To explore the correlation between protein tyrosine kinase 7 (PTK7) and coronary heart disease (CHD) and its diagnostic value. Methods Target genes were obtained through the Gene Expression Omnibus (GEO) database. StataSE15 was used to find the total standardized mean difference (SMD) of PTK7 and plot the summary receiver operating characteristic (SROC) curve. Next, reverse transcription quantitative polymerase chain reaction was used to verify the expression of PTK7 in CHD and non-CHD population samples and search for CHD-related single-cell RNA sequencing data from GEO to analyze the expression of PTK7 in different cells. The upstream transcription factor (TF) of PTK7 was predicted by the Cistrome Data Browser database. Moreover, enrichment analysis of the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed on the differentially co-expressed genes. Results By calculating the SMD of PTK7, it was found that PTK7 was highly expressed in the peripheral blood leukocytes (PBL) of patients with CHD (total SMD=0.1,5% confidence interval=0.17~1.45). The population sample validation confirmed the above results. When SROC was plotted, the area under the curve (AUC) was 0.79, indicating that PTK7 has the ability to distinguish between CHD and non-CHD. The single-cell RNA sequencing results showed that the expression ratio of PTK7 was relatively low in different cells of normal peripheral blood. In addition, potential upstream TFs of PTK7 were predicted through the ChIP-seq database, where it was found that IRAK1, SNAI2 may be positive upstream TFs of PTK7, and EP300, NIPBL may be negative upstream TFs of PTK7. Conclusion Highly expressed PTK7 in PBL is positively correlated with the pathogenesis of CHD, demonstrating that PTK7 has definite diagnostic value on CHD.