血管内血栓形成及溶栓后再闭塞的动物模型
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An Animal Model in Inducing Introarterial Thrombosis with Subsequent Reocclusion after Thrombolysis
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    本文采用高脂饲养,机械损伤及电刺激方法,加用尿激酶溶栓,制备了兔股动脉血栓形成及溶栓后再闭塞动物模型。结果表明:所处理血管电刺激2h内血栓形成达完全闭塞。溶栓再通后2h,血管再闭塞率达50%。在血栓形成及再闭塞时,血小板聚集率,血栓素A2含量及纤溶酶原激活物抑制物活性明显增加,而前列环素含量及纤溶酶原激活物活性显著降低。提示,本模型血栓形成率高,再闭塞率及血栓成分近似于冠心病,且方法简便、易行。

    Abstract:

    Model of the femoral arterial thrombosis was produced in all 20 rabbits by feeding high cholesterol,manipulating with machanical and electrical damage.Urokinase was then given and reocclusion will be observed subsequently,2 h after recanalization,50% of arteries were reoccluded assessed by angiography. Intimal injury,adhered thrombus containing platelet riched mixed cells were confirmed by pathological examination.During the process of thrombosis and reocclusion, platelet aggregetion rate content of plasma thromboxane A2 and activity of plasminogen activator inhibitor weremarkably increased. Whereas the level of prostacyclin and activity of plasminogen activator significantly decreased.Conclusion:rate of thrombus formation is high the ingradient of thrombus and the rate of reocclusion are closed to human coronary disease.The endothelial injury and activation of platelet being the basis of changs of vessel active substance corspond to the characteristic of formation of coronary thrombosis and reocclusion after thrombolysis.Since the method of preparation is easy and feasible,it wiil be a valuable model for studing some new drug in preniting the reocclusion after thromblysis.

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熊一力,赵华月.血管内血栓形成及溶栓后再闭塞的动物模型[J].中国动脉硬化杂志,1994,2(4):140~144.

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  • 收稿日期:1994-10-11
  • 最后修改日期:1994-11-04
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