The sera of hypercholesterolemia (HC)from people of Beijing and Guangzhou were incubated with U937 cells respectively for 40 hours,then binding rate between U937 cell and fluorescencelabeled high density lipoprotein(HDL) were detected on cell sorter. The result showed that the binding rate of cells treated with HC from Beijing was significantly higher(2～20 fold) than that from Guangzhou, of which the binding rate of cells was similar to control cells(treated with normal lipid sera). Apolipoprotein E(Apo E)and its receptor pathway did not play an important role in the binding process,HDL3 is major subclass of HDL which bound to U937 cells,These findings indicated that HC from Beijing has a role to stimulate monocyte cell to take lipids, while HC from Guangzhou doesn't. The difference should exist in plasma component between the masses of Beijing and Guangzhou with HC, which may contribute to high incidence of coronary heart disease in Beijing compared with that in Guangzhou.