Aim Platelet derived growth factor (PDGF) plays important roles in the development of atherosclerosis.In order to block the effects of PDGF, PDGF receptor binding domain was fused with the hepatitis B core antigen (HBcAg) to form a fusion protein. This fusion protein can be used as a vaccine for the prevention of atherosclerosis in animal models. In this paper we report only the cloning of the fusion protein gene. Methods The gene of PDGF receptor binding domain, a 13 iner peptide, ANFLVWEIVRKKP, with 2 linkers (for Ndel and EcoRI) was synthesized chemically and cloned into a vector PBS. The HBcAg gene was obtained with polymerase chain reaction (PCR)technique and fused with the 13 iner peptide gene-pBS at the EcoRI and BamHI sites. Results A DNA fragment of about 600 hp in size was obtained. DNA sequencing analysis showed that the sequence of the 13iner peptide gene and the reading frame of the fused gene were both correct. Conclusion The gene of the fusion protein of the 13 iner peptide with HBcAg was successfully cloned in the vector PBS.