Aim To dissect the contribution of each repeat of the ligand binding domain of the VLDL receptor to ligand binding. Methods Several VLDLR recombinants lacking different repeat(s) were constructed by the method of oligonucleotide directed mutagenesis and then introduced into CHO cells by transfection. The ability of the mutant receptor to bind with β VLDL was measured. Results The result of the binding experiments showed that repeat 1 and 2 are the most important repeats required for β VLDL binding. Conclusion These results lay the foundation of VLDLR structure and function study.