Aim To introduce a multilocus genotyping assay for candidate genotypes of cardiovascular disease (CVD) established by Roche Molecular Systems (RMS). Methods By applying the regents, materials and the protocol provided by RMS, genotyping was done for the Chinese from different regions (Beijing and Hong Kong) and a French population. Results By comparing the allelic frequencies between the Chinese from different regions and between the Chinese and the French populations, no significant difference was found for each allelic frequency between the two Chinese groups, but some allelic frequencies significantly differed between the Chinese and the French populations. Conclusions This multilocus genotyping assay established by RMS is a rapid, convenient and efficient method in determining the genotypes possibly related to the development of CVD in a large scale population study for the genetic risk of CVD.