Aim To investigate the long term effect of dietary fiber complex (DFC) B at different levels on cholesterol metabolism in hypercholesterolemic rats. Methods 84 healthy SD rats were fed with diet rich in lipids and hypercholesterolemic animal models were established. The rats were randomly divided into 7 groups: hypercholesterolemic control group(HC), 4%DFC group, 8%DFC group, 16%DFC group, 32%DFC group and 64%DFC group. Wheat fiber was used in the hypercholesterolemic control group and rats fed with normal diet were used as control group. Different levels of DFC B were given in the experimental groups. Results The concentrations of plasma total cholesterol (TC),low density lipoprotein cholesterol (LDLC) were all significantly lowered by DFC B at different levels and those of high density lipoprotein cholesterol (HDLC) were significantly elevated compared with hypercholesterolemic control group (p<0.05);Dose response relationship was observed in the effect of DFC on plasma TC. Conclusion DFC composed mainly of Hippophae rhamnoides L bran, oat bran and guar gum can reduce the plasma TC, LDLC and raise HDLC effectively in hypercholesterolemic rats, thus could be used for preventing and therapying atherosclerosis and coronary heart disease.