巨噬细胞源性泡沫细胞适配子PM1的特异性
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Research on the Specificity of PM1 Aptamer About THP-1 Macrophage Derived Foam Cells
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    目的研究特异性寡核苷酸适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变结合的特异性,为动脉粥样硬化的体内靶向治疗提供实验依据。方法对适配子PM1进行FITC标记,荧光显微镜分别观察适配子与血管平滑肌细胞、巨噬细胞、内皮细胞、巨噬细胞源性泡沫细胞结合情况。采用高脂方法建立兔动脉粥样硬化模型,利用PCR方法和荧光显微镜观察适配子与动脉粥样硬化病变结合情况。结果适配子PM1与巨噬细胞源性泡沫细胞和动脉粥样硬化病变组织高度特异性结合,但不结合血管平滑肌细胞、巨噬细胞、内皮细胞和正常动脉组织。结论筛选出的适配子PM1能特异性结合巨噬细胞源性泡沫细胞和动脉粥样硬化病变。

    Abstract:

    Aim To observe the binding specificity of aptamers and THP-1 macrophage derived foam cells and atherosclerotic lesions.Methods The FITC-labeled PM1,binding to THP-1 macrophages,vascular smooth cells,endothelial cells and THP-1 macrophage derived foam cells were observed by fluorescence microscopy respectivly.Model of atherosclerosis was formed by high-fat feeding rabbit methods.The binding of aptamers and atherosclerotic lesions were detected by fluorescence microscopy and PCR.Results PM1 didn't bind to THP-1 macrophages,vascular smooth cells and endothelial cells but bind to THP-1 macrophage derived foam cells and atherosclerotic lesions.Conclusions we have obtained PM1 specifically binding to THP-1 macrophage derived foam cells and atherosclerotic lesions.

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严鹏科, 汪江波, 张慧, 段才闻, 李世煌.巨噬细胞源性泡沫细胞适配子PM1的特异性[J].中国动脉硬化杂志,2011,19(5):405~408.

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  • 收稿日期:2011-04-17
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