AimTo determine the effect of oxLig-1, synthesized by an chemical method, on the monocyte chemoattractant protein-1 (MCP-1) expression in human THP-1 monocytes.MethodsoxLig-1 was identified by mass spectrometry and nuclear magnetic resonance (NMR) analysis.Human THP-1 monocytes were treated with different concentration (15, 30 and 60 mg/L) of oxLig-1, the apoptosis of the cell was detected by flow cytometry using Annexin-V and PI.The mRNA and protein levels of MCP-1 were measured by RT-PCR and ELISA methods, respectively.ResultsThe structure of synthetic oxLig-1 identified by LC-MS and NMR analysis showed the same as the natural structure of oxLig-1.There were no differences in THP -1 cell apoptosis, as evidenced by FCM at a high concentration of oxLig-1 (60 mg/L).The mRNA and protein expressions of MCP-1 were increased in a dose-dependent manner of oxLig-1, and the maximum value was observed at the concentration of 40 mg/L.ConclusionThe oxLig-1 could upregulate the expression level of MCP-1 in human THP-1 cells.