Aim To investigate the expressions of CD54, CD106 on the surface of endothelial cells after interaction with platelets from the patients with coronary heart disease (CHD).Methods To draw blood from acute coronary syndrome patients (acute coronary syndrome group), patients with stable angina (stable angina group) and healthy people (control group), the platelets were extracted and interacted with endothelial cells. Then the expression of CD54, CD106 on the surface of endothelial cells were tested.Results The expression level of CD54 on the surface of endothelial cells in acute coronary syndrome group were obviously higher than in control group (P<0.01), and higher than in stable angina group (P<0.05) but no difference was found in CD54 between control group and stable angina group. The expression level of CD54 was significantly and positively related with the markers of myocardial necrosis such as initial troponin I and initial creatine kinase-MB (CK-MB). There were no obvious differences of the expression level of CD106 among each group (P>0.05).Conclusions After interaction of endothelial cells with platelets, the expression level of CD54 on the surface of endothelial cells from the patients with acute coronary syndrome was obviously increased, and was associated with the markers of myocardial necrosis. We can infer that CD54 is a very important index which can be used to predict the occurrence and severity of CHD.