Aim To explore the effect of miR-152 on low density lipoprotein receptor （LDLR） expression and hepatocyte lipid uptake. Methods Bioinformatics websites predicted the combination of miR-152 with LDLR 3’UTR. Luciferase reporter assay confirmed the binding of miR-152 to LDLR 3’UTR. LDLR expression was measured by Western Bolt,and intracellular lipid droplet stained with oil red O in HepG2 cells transfected miR-152 mimic and inhibitor. Results MiR-152 was bound to the 872-878 sites within human LDLR 3’UTR,furthermore,their binding free energy was very low. MiR-152 obviously inhibited the luciferase activity and hepatocyte LDLR expression. Lipid uptake was dramatically suppressed in HepG2 cells treated with miR-152. The exactly opposite results were observed by anti-miR-152 treatment. Conclusions MiR-152 targets LDLR expression and inhibits hepatocyte lipid uptake.