Damage to organelles plays a significant role in myocardial ischemia/reperfusion injury, which results in the dysfunction of mitochondria and other related organelles. The communication between mitochondria and other organelles can also affect the development of myocardial ischemia/reperfusion injury. For instance, the mitochondria-associated endoplasmic reticulum membrane provides a “seamless connection” and regulates the exchange of organelles and metabolites (such as ions, lipids and proteins) between the mitochondria and the endoplasmic reticulum, which subsequently affects myocardial ischemia/reperfusion injury. However, there is a lack of studies regarding the interaction between mitochondria and related organelles, which is a critical component in triggering myocardial ischemia/reperfusion injury. Therefore, this article describes the role of mitochondrial crosstalk with endoplasmic reticulum, lysosomes and nuclei in myocardial ischemia/reperfusion injury, and aims to provide a theoretical basis for targeting mitochondrial crosstalk with other organelles in the treatment of myocardial ischemia/reperfusion injury.