In present experiment the temporal increase of the calcium level both in cytoplasma and nuclear of single culture endothelial cell was observed after stimulation of tumor necrosis factor-a (TNFa, 1MIU·L-1), however, the increase of calcium level was more significant in nuclear and decreased slowly. The calcium level was not changed obviously in white cell after stimulation of TNFa in same dosage. The calcium level both in endothelial cell and white cell increased rapidly and ten decreased less than the original level alter stimulation of phorbol myristate acetate (3. 5nmol·L-1 ) which is a activator for protein kinase C (PKC). Finally, it was found that the nuclear calcium of endothelial cell was output entirely, but there was calcium reside in the n[lclear of white cell. The results suggested that the effects of TNFa stimulation are different on the different kinds of target cells. the changes of calcium level are different in different region of cell,and the PKC plays an important role in the calcium output from inside of the cell.but it may don't participate in the offect of TNFa on the cell calcium. Investigating the calcium change in the single endothelinalcell should benefit the research on the role of Ca²⁺ in the pathogenesis of atherosclerosis.