In this study, a typical model of macrophages-derived foam cell was founded by peritoneal macrophage of C57 BL/6J mouse, which is susceptible to atherosclerosis, incubated with 10 mg·L-1 oxidized low density lipoprotein. Comparing with macrophages of Swiss strain mouse, these results showed the cultured foam cell-derived from C57 BL/6J mouse macrophages continued a large of lipid particles and intracellular total cholesterol increased in the cells, in which cholesteryl ester occupied more than 50% of total cholesterol. It corresponded to the typicalcharacteristics of foam cells. This model suppled a reliable pathological cell tool for the study of atherosclerosis.