Aim To study the existence of high density lipoprotein (HDL) receptor in the cell membrane, and this may by provided the evidence for study of HDL receptor function.Methods HDL binding protein was prepared from fresh swine adrenal cortical cell membranes, and purified with affinity chromatography. The soluable membrane protein was analyzed by ligand blotting.Results The dot blotting showed that the membrane protein possessed high affinity with HDL, and western blotting suggested that the molecular weight (MW) of the binding protein by affinity chromatography is 77 kDa. The characters of the HDL binding protein was measured by biotin-avidin enzyme linked immunosorbent assay (BA-ELISA ): Kd= 21. 6 mg/L, Bmax= 678. 2 μg/g protein. Conclusion It is showed that the condition of affinity chromatography was moderate, and the purified swine adrenal cortical cell membrane possessed high affinity with HDL.