Aim: To determine the genetic polymorphism and phenotypic distribution of healthy human sera apolipoprotein A Ⅳ in Chinese.Methods: The sera from 180 normal individuals has been detected to determine apolipoprotein A Ⅳ by isoelectric focusing (IEF ) techniques and immunoblotting.Results: The gene frequencies of the two common alleles, apolipoprotein A Ⅳ*1 and* 2, were 99. 44%and 0. 28%, respectively. The distribution of the two common phenotypes, apolipoprotein A Ⅳ -1/1 and AⅣ-2/1, were 98. 89% and 0. 56%, respectively.Conclusion: The statistically difference was significant in apolipoprotein A Ⅳ allele frequences and phenotype distribution between normal Chinese and American and European (P<0. 05). There is no significant difference in apolipoprotein A Ⅳ allele frequencies and phenotype distribution between normal Chinese and other Asian (P>0. 05 ). These results suggest that apolipoprotein A Ⅳ polymorphism is different with different regions and races.