Time-Effective Relation of Proliferation of Aortic Smooth Muscle Cells after Lo cal Delivery of C-sis Proto-oncogene Antisense Oligodeoxynucleotides in Injured Aorta of Rabbits
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    Abstract:

    Aim To study the inhibitory effect of locally delivered c-sis proto-oncogene antisense oligodeoxynucleotide (ODN) on aortic smooth muscle cells (ASMC)proliferation and neointimal thickening. following balloon angioplasty in the rabbit aorta at differential time.Methods 60 male New Zealand, White Fabbits were treated with local delivery of c-sis antisense ODN,c-sis sense ODN, or 0. 9% salt solution via balloon catheter following aorta balloon injury, ASMC proliferation and area in neointima was assessed with immunohistochemistry and image analyzer at 3, 7, 14, 28 days after local delivery.Results ASMC proliferation in neointima were reduced 69. 8%. 59 %, 51. 9%, 47. 8% in c-sis antisense ODN-treated group compared to c-sis sense ODNtreated group at 3, 7, 14, 28 d following aortic injury respectively; and the inhibitory rate of neointimal hyperplasia was 48. 9%, 77. 8%, 72. 6%, 70. 8% at the same time respectively.Conclusion The locally deliveried C-sis antisense ODN inhibited ASMC proliferation and neointimal thickening following rabbit aortic injury, the inhibitory effect was the highest at 3 d and 7 d, then reduced slowly. and caused by antisense mechanism.

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TANG Qi-Dong; LIN Shu-Guang, LIN Qiu- Xiong. Time-Effective Relation of Proliferation of Aortic Smooth Muscle Cells after Lo cal Delivery of C-sis Proto-oncogene Antisense Oligodeoxynucleotides in Injured Aorta of Rabbits[J]. Editorial Office of Chinese Journal of Arteriosclerosis,1998,6(3):206-210.

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  • Received:June 19,1998
  • Revised:August 19,1998
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