The Effect of Oxidized and Normal Very Low Density Lipoprotein on Lipid Accumulation in Porcine Aortic Smooth Muscle Cells
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    Abstract:

    Aim To observe the different effects of oxidized very low density lipoprotein(ox-VLDL)and normal VLDL(n-VLDL)on lipid accumulation in smooth muscle cells(SMCs)and attempt to explore receptor pathway of ox-VLDL uptaken by SMCs.Methods The same concentration of ox-VLDL or n-VLDL was incubated with porcine aortic SMCs for 48 hours,total cholesterol(TC)and triglyceride (TG) of the cells were extracted and determined.SMCs were incubated at 37℃ in RPIM 1640 contain-ing different concentration of FITC-ox-VLDL or FITC-n-VLDL for 4 hours,the cells were washed with phosphate buffered saline and the cell surface bound lipoproteins were determined by fluorospec- trometry.Competition experiments were performed as above.except for adding different concentration of unlabeled lipoproteins into the medium.Results ox-VLDL or n-VLDL(200mg/L)was in-cubated with porcine aortic SMCs for 48 hours,caus-ing a 3.2- and 10.6- fold increment of cellular TG in SMCs respectively(p<0.01).less TG was ac cumulated with ox-VLDL as compared with n-VLDL (P<0.01).Scatchard plot of the data of binding ex-periment showed that the Kd of the binding of ox-VLDL or n-VLDL on SMCs were 48.82mg/L and 37.17mg/L respectively,and the Bmax was 2067 μg ox-VLDL/g cell protein and 2596μg n-VLDL/g cell pro-tein respectively.nLDL competed with FITC-ox-VLDL on binding to SMCs as efficient as ox-VLDL,however,acetyl LDL inhibited only 10%.Conclusion More TG was accumulated in cells ex-posed to n-VLDL(P<0.01).FITC-ox-VLDL and FITC-n-VLDL were taken up by SMCs via saturable mechanism with low affinity.The Kd of the binding of ox-VLDL on SMCs was greater than that of n-VLDL and the Bmax was lower than that of n-VLDL.nLDL competed with FITC-ox-VLDL on binding to SMC as efficient as ox-VLDL,acetyl LDL exhibited low competitive effect,indicating that,ox-VLDL was taken up by SMC via LDL or VLDL receptor.

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YANG Yu-Hong;FENG Zong-Chen;WANG Chun-Ben;JIANG Wei-Guo, ZHAO Xiao-Song. The Effect of Oxidized and Normal Very Low Density Lipoprotein on Lipid Accumulation in Porcine Aortic Smooth Muscle Cells[J]. Editorial Office of Chinese Journal of Arteriosclerosis,1998,6(4):305-309.

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  • Received:June 26,1998
  • Revised:November 14,1998
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