Aim In order to see the effects of continued and stable expressive antisense c-myc on the apoptosis of vascular smooth muscle cell(VSMC) . Methods Three recombinant retroviral expressive vectors,aM1,aM2 and aM3,which respectively loading reverse fragments of exon 1,2 and 3,were transferred into rat artery SMC and assayed 1 month after their stable expression. Results Apoptosis ratioes of aM3 group were increased to 360%,while those of aM1 and aM3 groups were significantly decreased. In addition,SMC transfected with aM1 and aM3 (especially aM3),emerged morphological features of apoptosis and autophagy,while aM2 commonly appeared bunches of myofilament in electron microscopy. Conclusion Stable expression of antisense c-myc can induce SMC into apoptosis in vitro, and aM3 has the most powerful ability to induce apoptosis and autophagy.