Aim To investigate the effect and significance of glucose and insulin on phospholipase A 2 (PLA 2) activities of vascular smooth muscle cells(VSMC). Methods VSMCs in rats were incubated with the DEME which had different concentration glucose or/and insulin for 72 h PLA 2 activities were detected with [ 3 H]-Oleate radioactivity(cpm). Results PLA 2 activities increased with high concentration of glucose or/and insulin and there were dose-dependent relationship. Conclusion The increased PLA 2 activities were associated with atherosclerosis in diabetes, so treatment with PLA 2 inhibitor could prevent the pathological progress of athcrosclerosis in diabetes.