Aim To explore the correlation between CGG triplet repeat polymorphism on the 5' non-coding region of very low density lipoprotein receptor (VLDLR) gene and coronary heart disease (CHD) in Chinese Han nationality, 235 subjects with no blood links in Hubei region, including 75 cases with CHD and 160 healthy controls were studied. Methods Polymerase chain reaction (PCR)-denature polyacrylamide gel electrophoresis-silver stain technique was employed to determine the CGG triplet repeat polymorphism of VLDLR gene. Results The Chinese Han nationality in Hubei region has VLDLR gene polymorphism, 4 alleles with 5, 8, 9, 11 CGG triplet repeats and 8 genotypes with 5/5, 5/8, 8/8, 8/9, 9/9, 5/9, 8/11 and 5/11 were detected. There were no statistically significant differences in VLDLR gene allele and genotype frequencies between CHD and normal controls (P>0.05). Conclusions There is VLDLR gene polymorphism in the Han Chinese in Hubei region and such polymorphism has ethnic difference. CGG triplet repeat polymorphism of VLDLR gene has no correlation with CHD. It is not an independent risk factor