Aim To establish a direct, accurate method for detecting the tetranucleotide repeat genotype of human low density lipoprotein receptor related protein(LRP). Methods LRP was amplified by using polymerase chain reaction (PCR). The PCR products were subjected to electrophoresis on 6% denature polyacrylamide gels. Sequences of LRP were analysed by direct sequencing of PCR products. Results Using this method, we detected the LRP genotypes: 5/6, 6/6, 5/5 and two allelic genes 5, 6. The results were in agreement with sequence analysis. Conclusion LRP genotype can be determined directly and accurately. It is a tool for the investigation of large-scale population and study of cardio-cerebro vascular diseases(CCVD).