Aim This study was to investigate p57kip2 gene expression in hyperplastic or hypertrophic vascular smooth muscle cells (VSMC) stimulated by platelet-derived growth factor (PDGF-BB) and angiotensinⅡ(AngⅡ). Methods Rat aorta media smooth muscle cells were cultured. PDGF-BB and AngⅡwere added into serum-free medium at a concentration of 20 μg/L and 1 μmol/L, respectively. VSMC were harvested after stimulated for 24 h. Protein level of p57kip2 wAs investigated with Western blot analysis and p57kip2 gene mRNA wAs determined by microarray. Results Both p57kip2 mRNA and protein level were higher in PDGF-BB stimulated VSMC than those in AngⅡstimulated ones. The p57kip2 protein level of VSMC was similar in AngⅡ stimulated group as compared with serum-free control. Conclusions There is increased expression of p57kip2 protein during VSMC proliferation stimulated by PDGF-BB, which may prevent VSMC from overhyperplasia.