Aim To investigate the relationship between the promoter region of Bβ fibrinogen (Fg) gene polymorphism 148C/T, 455G/A and coronary heart disease (CHD). Methods By Polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) restriction enzyme digestion methods,the genotypes and allele frequencies of Bβ fibrinogen gene polymorphism 148C/T, 455G/A were determined in 148 CHD patients and 173 controls. Result Fg Bβ 148T, 455A allele frequencies are higher in CHD group than that in control group (p<0.05). Both heterozygous genotype frequencies of 148CT and 455GA are higher in CHD group than that in control group. The plasma Fg levels have differences among genotypes: 148CC, 455GG< 148CT, 455GA< 148TT, 455AA respectively. The plasma Fg levels are higher in CHD group than that in control group, when compared the same genotype, CHD group Fg levels also higher than that of in control group. Conclusion The promoter region of Bβ Fg gene polymorphism 148C/T, 455G/A are associated with the development of CHD, and at same time impact on the plasma Fg concentration. The polymorphism 148C/T and 455G/A are complete linkage disequilibrium in Southern Chinese.