Aim To study effect of homocysteine (Hcy) on taurine uptake in cultured rat vascular smooth muscle cells (VSMC). Methods In cultured VSMC of rats, cellular 3 H taurine uptake were determined by 3 H taurine incubation. Taurine transport velocity was measured by radio ligand method. Results Hcy (10～500 μmol/L) could inhibit taurine transport in a dose dependent manner. As compared to control group, 100 and 500 μmol/L Hcy significantly decreased taurine transport. The maximal transport velocity (Vmax) decreased by 31% (p<0.05) and 51% (p<0.01), respectively. There were no significant changes in the Km value. 5 mmol/L H 2O 2 inhibited taurine transport. Vmax decreased by 48% (p<0.01), but Km value increased by 45% (p<0.01). Taurine could increase taurine transport that Hcy inhibited. Conclusions Hcy could inhibit taurine transport; The exogenous taurine could efficiently inhibit taurine transport dysfunction by Hcy induced.