Aim In order to study the relationship bewteen activity of NF-κB and the expressions of monocyte chemotactic protein-1 (MCP-1) and protein kinase C (PKCα) and atherosclerosis (As) plaques of different stages. Methods Using electrophoretic mobility shift assay (EMSA), immunohistochemitry and in situ hybridyzation,We study activity of NF-κB and the expressions of monocyte chemotactic protein-1 (MCP-1) and protein kinase C (PKCα) varied in atherosclerosis (As)plaques of different stages. Results The resultes confirmed that the activity of NF-κB as well as the expressions of monocyte chemotactic protein-1 (MCP-1) and protein kinase C (PKCα) in As plaque was increased in a time dependent manner as compared with those in control group (p<0.05). Which was consistent with the increaceing of activity of NF-κB and up-regulated in the expressions of MCP-1and PKCα. Conclusion All of these indicated that NF-κB activation, the up-regulated expressions of MCP-1and PKCα may take part in pathogenesis and development of As.