Aim To study the change of the gene expression pattern of vascular endothelial cells induced by oxidized low density lipoprotein. Methods After human umbilical vein endothelial cells was exposed to oxidized low density lipoprotein(100 mg/L) for 24 h, genes expression was screened with a complementary DNA microarray representing 4 000 different human genes and 96 control genes. Results There were 1 down regulated gene and 3 up regulated genes. The down regulated gene was ubiquitin activating enzyme E1. The up regulate genes were as follows: serum and glucocorticoid inducible protein kinase (SGK), heat shock protein 70 (HSP70), and KM 102 derived reductase like factor (KDRF). Conclusions Oxidized low density lipoprotein could induce the change of gene expression pattern of endothelial cells. It was the first time to find that oxidized low density lipoprotein could induce the change of the gene expression of SGK, HSP70 and KDRF. All the changed genes were related to the stress stimulation. These changes might be related to the dysfunction of endothelial cells induced by oxidized low density lipoprotein.