Aim To explore the protective effect of probucol on the human umbilical vein endothelial cell (hUVECs) injured by lysophosphatidylcholine. Methods Confluent hUVECs were incubated with Probucol for 24 h and consequently exposed to lysophosphatidylcholine. Lactate dihydrogenase (LDH), plasminogen activator inhibitor 1 (PAI 1)and tissue plasminogen activator(t PA) activity was measured in cell medium and lysate. Endothelial nitric oxide synthase (eNOS) activity and nitric oxide (NO)production were assessed by endothelial nitric oxide synthase and nitric oxide kits. Results Lysophosphatidylcholine enhanced markedly PAI 1, LDH activity and decreased nitric oxide bioavailability via the inhibited eNOS activity (p<0.01). These effects were attenuated by administration of different dose of probucol (10～50 mmol/L). Conclusions Lysophosphatidylcholine could injure hUVECs and inhibit eNOS and t PA activity directly. Probucol might play a protective role in hUVECs injured by lysophosphatidylcholine.