Effect of Simvastatin on Cluster of differentiation 40 Expression Induced by Tumor Necrosis Factor-α/Interferon-γin ECV-304 Cell
CSTR:
Author:
  • Article
  • | |
  • Metrics
  • | |
  • Related
  • |
  • Cited by [0]
  • | |
  • Comments
    Abstract:

    Aim To investigate the effect of simvastatin on CD40 expression induced by tumor necrosis factor α (TNF α) and interferon γ (IFN γ). Methods Human umbilical vein endothelial cells (hUVECs) cell line (ECV 304) were cultured with simvastatin (0~10 μmol/L) , and stimulated with 100 kU/L TNF α and 1 500 kU/L IFN γ. We detected CD40 expression levels in hUVECs by reverse transcription polymerase chain reaction (RT PCR) and flow cytometry (FCM) analysis. Results In ECV 304, exposure to TNF α (100 kU/L) plus IFN γ (1 500 kU/L) resulted in a marked increase in CD40 expression; CD40 mRNA expression reached a maximum at 9 h, and protein at 20 h. Simvastatin attenuated the expression of CD40 exposed to TNF α (100 kU/L) plus IFN γ (1 500 kU/L) in a dose dependent manner, with a maximal effect at concentration of 10 μmol/L. On mRNA level, relative intensity quantification compared with basal were 90.9%±38.8% vs 228.0%±49.6% (p<0.05); on protein level, CD40 + cells ratio were 20.1%±1.3% vs 54.1%±6.1%(p<0.05). Conclusions Simvastatin can inhibit CD40 expression induced by TNF α/IFN γ. These results may have important implications for mechanism of anti inflammatory and immunological effect of statins.

    Reference
    Related
Get Citation

ZHANG Min, CHEN Zhen Yue, LU Guo Ping,, WU Chun Fang. Effect of Simvastatin on Cluster of differentiation 40 Expression Induced by Tumor Necrosis Factor-α/Interferon-γin ECV-304 Cell[J]. Editorial Office of Chinese Journal of Arteriosclerosis,2003,11(3):234-237.

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:September 27,2002
  • Revised:September 27,2002
Article QR Code