Aim To establish a new animal model of the human type 2 diabetes mellitus complicated with atherosclerosis and investigate the effects of high fat/high sucrose feeding on glucose and lipid metabolism in guizhou minipigs. Methods Minipigs were fed with high fat/high sucrose diet (HFSD) and the levels of fasting blood glucose, insulin, total cholesterol, triglycerides were measured. Dysfunction of the pancreatic β-cell were observed by immunohistochemistry. The aortic fatty streak-lesions were quantified following lipid staining with Sudan IV. Lipid droplets in liver were observed by Oil red O staining. Results During the feeding period, HFSD induced hyperglycemia, hypercholesterolemia and hypertriglyceridemia. At the end of 8 months, in HFSD-fed animals, the adipocytes were hypertrophic. Injured pancreatic β-cells were observed. The aortic fatty streak-lesions were clearly presented in the animals' aortas. Extensive fat deposited in the liver were observed. Conclusion Our study successfully established a new animal model that feeding minipigs with high fat/high sucrose diet without adding dietary cholesterol can induce insulin resistance, mild diabetes, and atherosclerotic lesions. High fat/high sucrose diet-fed minipigs may be a good animal model for research on the treatment of diabetic dyslipidemia complicated with atherosclerosis.