Aim To understand the relationship of interleukin-6-gene 174G/C polymorphism and insulin resistance in Han people in south China. Methods Interleukin-6-gene 174G/C polymorphism were detected in 69 patients with high insulin resistance index and 69 control with low insulin resistance index by polymerase chain reaction-restriction fragment length polymorphism analytical method, meanwhile, combined with blood pressure, plasma glucose, blood fat, et al to undertake cross-sectional study. Results In Han people in south China, interleukin-6-gene 174G/C polymorphism main was G/G homogenotype and distribution frequency was 136/138 (98.55%), G/C hetrogenotype distribution frequency was 2/138(1.45%), C/C mutant genotype had not been found. There was no relationship between the kinds of genotype and insulin resistance index. Conclusion Maybe there is no relationship between interleukin-6-gene 174G/C polymorphism and insulin resistance in Han people in south China.