Aim To investigate the effect of platelet activating factor receptor (PAFR) antagonist on angiogenesis in plaque and lesion area of apolipoprotein E-deficient mice (apo E -/-). Methods 8-week-old male apo E -/- mice were fed a standard chow diet for 24 weeks to develop advanced lesions. Mice were then separated into the following two groups (n=18) and treated for 8 weeks: (1) chow diet with regular water, (2) chow diet with WEB₂086 in the water (4.3 mg in 100 mL). Whole-Mount CD31 Stain and Plaque-Associated Sprouting have been used to estimate angiogenesis in plaque of aorta, Sudan Ⅳ staining to measure lesion area. Results Administration of WEB 2086 did not affect the lipid composition of mouse serum. However, lesion area was r significantly educed (31.4%±9.7% vs 17.5%±6.3%,p<0.01). The mean density of intimal capillarie of atherosclerotic plaque (34.6%±10.2% vs 16.1%±6.7%,p<0.01) and the mean number of sprouts per ring were significantly reduced in the WEB₂086 group (172.3±40.6 vs 73.1±24.9,p<0.05). Conclusions These studies provide evidence that WEB₂086 can inhibits angiogenesis in atherosclerotic plaque and reduce lesion area of apolipoprotein E-deficient mice.