Aim Progressive vascular disease is the leading cause of death in patient with diabetes mellitus. Advanced glycation end products (AGE) modified proteins are found in plasma and atherosclerosis lesion of diabetes mellitus patients. The study was conducted to elucidate the infection of AGE on expression of adhesion molecules E-selectin on human endothelial cell. Methods Human umbilical vein endothelial cell were coincubated in vitro with native human serum albumin(HSA), HSA modified with advanced glycation end products(AGE-HSA). The expression of adhesion molecule E-selectin was determined by immunofluorescen staining and flow cytometer analysis. Results E-selectin was not constitutively expressed on hUVEC. AGE-HSA enhanced the express of E-selectin on hUVEC in a time- and dose- dependent manner. HSA had no effect on the expression of adhesion molecule. Conclusion AGE up-regulates the expression of adhesion molecule on human endothelial cell and this can promote the infiltration of monocytes/macrophages in the intima and the formation of atherosclerosis.