Aim To observe the influence of Lacidipine and Amlodipine on tumour necrosis factor(TNF-a) induced expression of intercellular adhesion molecule-1(ICAM-1) in human umbilical vein endothelial cells(hUVEC).Methods Lacidipine and Amlodipine of different concentrations were incorporated in hUVEC using low density lipoprotein(LDL) as a carrier for 45 minutes respectively.Human recombinant TNF-a was then incubated with hUVEC for 6 hours.The expression of ICAM-1 in protein level and mRNA level was detected by flow cytometric technique and RT-PCR.Results Lacidipine,at any concentration,determined a dose-dependent significant decrease of the expression of ICAM-1.Amlodipine determined variable decreases of ICAM-1 at the intermediate and highest concentrations.Conclusion According to the results of flow cytometric technique and RT-PCR,Lacidipine and Amlodipine exert different effect on the TNF-a induced expression of ICAM-1 in hUVEC,which may be related with their different lipophilicity and antioxidant activity.