Aim To explore the influence of endothelin-1(ET-1) on the gene expression of human vascular endothelial cells and the molecular mechanism of vascular endothelial cells injury induced by ET-1. Methods ECV304 cells were cultured and the total RNA was extracted.The GEArry Q Series Human Endothelial Cell Biology Gene Array was used to analyze the differential expression of genes associated with the major functions of endothelial cells 12 hours after treated with ET-1. Results Compared with control group,expression of 53 genes was differentiated at the time point of 12-hour in endothelin group.Among them,expression of 22 genes including mainly antithrombotic and antioxidant genes was downregulated and that of 31 genes including mainly prothrombotic and inflammatory factor genes was upregulated. Conclusions ET-1 could injure human vascular endothelial cells by downregulating antithrombotic and antioxidant genes and upregulating prothrombotic and inflammatory factors genes