Aim To investigate effects of Propolis-ethanol extract on platelet adhesion to collagen function. Methods Intima-injury model was copied by using vitro perfusion.Platelet coverage on collegen was measured when shearing stress was 1000/s after blood passing collagen surface.We set three groups:negative control group(24% ethanol),experimental group(0.1 g/L,24% Propolis-ethanol extract),positive control group(0.1 g/L,ferulic acid). Results Platelet coverage in experimental group was decreased from 24.0% to 11.5%,there was significant difference than that in negative control group(p<0.01);There was no significant difference between experimental group and positive control group(p>0.05). Conclusions Propolis-ethanol extract could decrease the platelet adhesiveness area on collagen surface.