Aim To investigate the effect of fluvastatin on expression of matrix metalloproteinase-9 (MMP-9), tissue factor (TF) from the peripheral blood monocytes in patients with acute myocardial infarction (AMI). Methods Monocytes from 30 AMI patients and 30 control subjects were separated and added into two bores, one cultivated directly, one cultivated with fluvastatin (360 μg/L). The contents of MMP-9, TF were assayed by enzyme linked immunosorbent assay (ELISA). Results Compared with control noninterfering group, the productions of MMP-9, TF in AMI noninterfering group were increased significantly (351±141 μg/L vs 121±10 μg/L, p<0.01; 41.4±13.6 ng/L vs 25.5±5.4 ng/L, p<0.01). In AMI group, compared with the noninterfering group, the productions of MMP-9, TF in fluvastatin group have been decreased significantly (226±89 μg/L vs 351±141 μg/L, p<0.01; 31.4±11.2 ng/L vs 41.4±13.6 ng/L, p<0.05). Conclusion The productions of MMP-9, TF in monocytes from patients with AMI were increased. Fluvastatin inhibited the expression of MMP-9 and TF.