Aim To observe the protein expression of cyclophilin A in lipid-loaded macrophages induced by oxidized low density lipoprotein(ox-LDL) and its effect on cellular cholesterol accumulation. Methods RAW264.7 mouse macrophages were treated with 75 mg/L ox-LDL for 0,24,48 and 72 hours.Oil red O dye experiment was used to show the lipid droplets in lipid-loaded macrophages. High performance liquid chromatography(HPLC) analysis was peformed to determine the content of cellular total cholesterol(TC) and cholesterol ester(CE).Westernblot analysis was used to detect the protein expression of cyclophilin A.Then,75 mg/L Probucol was added in the cells treated with ox-LDL for 48 hours. Results With ox-LDL treatment after 48 hours,Oil red O dye showed a lot of lipid droplets accumulated in RAW264.7 macrophages,HPLC analysis showed the content of cellular TC and CE were increased greatly and Western-blot analysis showed the expression of cyclophilin A was significantly decreased.However,with Probucol treatment,cyclophilin A was greatly increased,the lipid droplets significantly decreased,and the content of TC and CE also decreased. Conclusion The protein expression of cyclophilin A was down-regulated in lipid-loaded macrophages while the content of cellular TC and CE were increased greatly.However,Probucol could up-regulate the cyclophilin A expression and decrease the cellular cholesterol accumulation.