Aim To research the hemodynamic property and the anti-calcification feature of bovine jugular vein conduit cross-linked by dye-mediated photooxidation. Methods Right ventricular outflow tract(RVOT) was reconstructed for 12 canines,7 with bovine jugular vein conduits cross-linked by dye-mediated photooxidation treatment,7 with bovine jugular vein conduits simply cross-linked by glutaraldehyde.After feeding 9 to 10 months,then the hemodynamic property was evaluated by echocardiography and heart catheter examination.Tissue calcium content was analyzed by flame atomic absorption spectrophotometer. Results Echocardiography revealed that the motion of the valvular leaflets in both groups of conduit that cross-linked by glutaraldehyde and cross-linked by dye-mediated photooxidation were satisfying.Heart catheter revealed the transvalvular pressure gradients of both studied groups were minimal.Walls of bovine jugular vein conduit that cross-linked by dye-mediated photooxidation treatment had less calcification than bovine jugular vein conduits that simply cross-linked by glutaraldehyde.Compared with the latter ones,the tissue calcium content of the formers decreased 65.6%.It is also found that the walls of the bovine jugular vein had far more calcification than the valves in both groups. Conclusions RVOT with bovine jugular vein conduits was reconstructed at 9～10 months,which hemodynamic property seems equivalent to pulmonary artery.The bovine jugular vein cross-linked by dye-mediated photooxidation treatment appears to have less calcification.