Aim To study the effects of tetramethylpyrazine(TMP) on calmodulin(CaM) and calcinuerin(CaN) in the proliferation of vascular smooth muscle cell(VSMC) induced by angiotensinⅡ(AngⅡ). Methods A cell proliferating model of VSMC induced by angiotensinⅡ(AngⅡ) was established;the varity of CaM and CaN activities affected by tetramethylpyrazine(TMP) at different time and different concentration was observed by enzyme reaction phosphorus measurement. Results Cell proliferation activity,CaM and CaN activities were increased significantly in VSMC proliferation induced by AngⅡ(p<0.01).While treated with TMP,the index were obviously reduced compared with AngⅡ group(p<0.01). Conclusions The VSMC proliferation induced by AngⅡ can be inhibited by TMP significantly,and the inhibiting mechanism of TMP may be related to inhibiting CaM and CaN activities then restraining the proliferation of VSMC in a dose and time-dependent manner.