Aim To investigate the effects of peptide specific to CCR5 on monocyte chemotaxis. Methods The effects of peptide on the binding of mAb 2D7 to monocyte were surveyed by flow cytometry; Competitive binding of peptide and RANTES on monocyte was measured through Quantikine Human RANTES kit; Chemotaxis assay of monocyte toward peptide was performed in vitro;Effects of peptide on atherosclerosis formation was detected in vivo in mice. Results Peptide could inhibit the binding of 2D7 and RANTES to monocyte (IC50 was about 3.98 μmol/L). In vitro,peptide showed no chemotactic effect to monocyte(P=0.074) and could inhibit chemotactic activity of monocyte toward RANTES,the cell number of migration in the peptide+RANTES groups (23±10) was lower than that of the RANTES groups(62±13). Peptide could impair the influx of monocyte to the aortic root in mice(p<0.05). Conclusion Peptide could target to monocyte and suppress chemotactic activity of monocyte toward RANTES and reduce atherosclerosis.