Aim To inquire into the relationship between single nucleotide polymorphisms (SNP) of thrombospondin-1 gene (TSP-1) and acute myocardial infarction (AMI). Methods Fragment of Exon thirteen in TSP-1 gene from 172 cases of AMI and 270 subjects without coronary heart disease were analysed by polymerase chain reaction and restriction fragment length polymorphism, and sequence analysis for confirmation. Results Of the 442 subjects participating in the study, only 6 of the heterozygotes and none of the homozygotes were detected for the 700S allele. No association of the N700S polymorphism with an altered risk of AMI was found in our study (GA vs AA: OR=3.19, 95%CI 0.578～17.61, P=0.160). Conclusion Our study suggested that the TSP-1 N700S polymorphism was rare and unrelated to AMI in the Chinese Han population. This study accumulated additional data on SNP in TSP-1 gene.